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chemiluminescence assay kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology chemiluminescence assay kit
    Chemiluminescence Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atp+chemiluminescence+assay+kit/ATP%2FADP+Ratio+Chemiluminescence+Assay+kit/pm42887618-139-11-15
    Average 94 stars, based on 16 article reviews
    chemiluminescence assay kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 106 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 10 6 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Incubation:

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 106 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 10 6 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Chemiluminescence Immunoassay:

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 106 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Article Title: Graphene Oxide Potentiates Induction Efficiency and Functional Maturation of Induced Mammary Epithelial Cells
    Article Snippet: Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM; high glucose), GlutaMAXTM, KnockOutTM Serum Replacement (KSR), phosphate-buffered saline (PBS), penicillin-streptomycin (Pen-Strep), NeurobasalTM Medium, KnockOutTM DMEM/F12, N2 Supplement, and B27TM Supplement were sourced from Gibco (Thermo Fisher Scientific, USA); trypsin-EDTA (0.25%), dimethyl sulfoxide (DMSO), 4% paraformaldehyde (PFA), and VPA were obtained from Sigma-Aldrich (Merck KGaA, Germany); RepSox was sourced from Selleck Chemicals (USA); the cDNA synthesis kit and Hoechst 33342 nuclear stain were acquired from Beyotime Biotechnology (China); graphite powder, potassium permanganate (KMnO 4 ), sodium nitrate (NaNO 3 ), and hydrogen peroxide (H 2 O 2 ) for GO synthesis were procured from Beyotime Biotechnology (China). .. ATP Chemiluminescence Assay Kit were sourced from Elabscience. ..

    Article Title: Rosmarinic Acid Targets AKR1B1 to Ameliorate Atherosclerosis via Vascular Endothelial Cell Energy Metabolism Regulation
    Article Snippet: .. HUVECs were seeded in 6 cm cell culture dishes at a density of 2.0 × 10 6 cells/dish and achieving a density of 70–80%, the cells were pretreated with 3 μM RA for one hour, followed by treatment with 100 μg/mL Ox-LDL for an additional 48 h. After incubation, cells were washed twice, and ATP levels were evaluated using the ATP chemiluminescence assay kit (E-BC-F002, Elabscience, Wuhan, China). .. Protein samples were prepared with radio-immunoprecipitation assay (RIPA) buffer (BOSTER, Biological Technology, Wuhan, China) supplemented with 0.1% protease inhibitor and 0.1% phosphatase inhibitor.

    Article Title: Integrated Transcriptomic and Metabolomic Analyses Reveal Acyl-CoA Dehydrogenase-Mediated Primordium Formation and Metabolite Synthesis in Cordyceps militaris .
    Article Snippet: .. ATP content was measured using an enhanced Elabscience ATP chemiluminescence assay kit (Elabscience, Wuhan, China). ..

    Article Title: Acupuncture Alleviates Allergic Rhinitis by Activating the AMPK / PGC ‐1α Pathway to Attenuate Mitochondrial Oxidative Stress and Restore Epithelial Barrier Function
    Article Snippet: Levels of malondialdehyde (MDA, #S0131S, Beyotime), catalase (CAT, #A007- 1- 1, Nanjingjiancheng), and superoxide dismutase (SOD, #S0101S, Beyotime) were then measured in both sample types using commercial assay kits according to the manufacturers' protocols. .. ATP content in mouse nasal mucosa tissues and HNECs was measured using an Enhanced ATP Chemiluminescence Assay Kit (#E- BC- F201, Elabscience, China) according to the manufacturer's protocol. ..

    Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission
    Article Snippet: .. Total ATP levels were then determined using the ATP Chemiluminescence Assay Kit (E-BC-F002, Elabscience), with measurements normalized to cell count. ..

    other:

    Article Title: AXL prevents amyloid-β-induced microglial ferroptosis by sustaining SLC2A3-mediated mitochondrial respiration.
    Article Snippet: The mouse 4-hydroxynonenal (4-HNE) assay kit (ml420008) was purchased from Enzyme-linked biotechnology (Shanghai, China).

    Article Title: AXL prevents amyloid-β-induced microglial ferroptosis by sustaining SLC2A3-mediated mitochondrial respiration.
    Article Snippet: 4-HNE levels in the culture medium were measured using a mouse 4-HNE assay kit (Shanghai Mlbio, ml42000).

    Cell Characterization:

    Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission
    Article Snippet: .. Total ATP levels were then determined using the ATP Chemiluminescence Assay Kit (E-BC-F002, Elabscience), with measurements normalized to cell count. ..



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    ( A ) Schematic of AK7 knockdown in ACC glutamatergic neurons using shRNA. ( B ) Western blot confirming effective AK7 knockdown in the ACC (* P = 0.0407, two-sample Student’s t test, n = 3 mice per group). ( C ) Knockdown of AK7 significantly increased PWT and PWL in CFA mice (* P = 0.0154, ** P = 0.0028, two-sample Student’s t test, n = 6 mice per group). ( D ) Representative action potential firing traces elicited by 150 pA depolarizing current injection. Scale bars: 50 mV, 100 ms. ( E ) Statistical comparison of AP peak amplitude between the two groups ( P = 0.2986, no significant difference, two-sample Student’s t test, n = 7 neurons per group). ( F ) Input–output curve showing the number of evoked APs under gradient injected currents (0–300 pA) (*** P < 0.0001, two-way repeated-measures ANOVA). ( G ) Quantitative statistics of resting membrane potential (** P = 0.0087, two-sample Student’s t test, n = 7 neurons per group) and rheobase current (** P = 0.0067, two-sample Student’s t test, n = 7 neurons per group) of ACC neurons. ( H ) Representative raw traces of sEPSCs recorded from ACC neurons in Scramble (blue) and AK7-KD (red) groups. Scale bars: 20 pA, 1 s. ( I ) Statistical analysis of sEPSCs amplitude (left, * P = 0.0153, two-sample Student’s t test, n = 7 neurons per group) and frequency (right, ** P = 0.0011, two-sample Student’s t test, n = 7 neurons per group) between two groups. ( J ) Cumulative probability distribution curves of sEPSCs amplitude (left) and inter-event interval (right) in Scramble and AK7-KD groups. ( K ) Immunofluorescence showing reduced c-Fos expression in the ACC after AK7 knockdown (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( L – N ) Fiber photometry showing decreased Ca²⁺ activity in ACC neurons following AK7 knockdown (* P = 0.0481, ** P = 0.0027, two-sample Student’s t test, n = 6 per group). ( O ) The Western blot experiment confirmed the expression of p-AMPK protein in the ACC brain region of mice (* P = 0.0299; 0.0469, one-way ANOVA with Tukey’s multiple-comparisons test, n = 5 mice per group). ( P ) Biochemical quantitative detection results of adenosine concentration in the ACC brain region of mice (** P = 0.0042; 0.0027, one-way ANOVA with Tukey’s multiple-comparisons test, n = 6 mice per group). ( Q ) Biochemical quantitative detection results of <t>ATP/ADP</t> ratio in the ACC area of mice brain (* P = 0.0215; 0.0413, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 mice per group). ( R ) Schematic of AK7 overexpression in ACC glutamatergic neurons. ( S ) Western blot confirming AK7 overexpression (* P = 0.0364, two-sample Student’s t test, n = 4 mice per group). ( T ) Overexpression of AK7 decreased PWT and PWL in CON mice (** P = 0.0046; 0.0011, two-sample Student’s t test, n = 6 mice per group). ( U ) Increased ACC c-Fos expression following AK7 overexpression (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( V – X ) Fiber photometry showing enhanced Ca²⁺ activity in the ACC after AK7 overexpression (** P = 0.0052, *** P = 0.005, two-sample Student’s t test, n = 6 per group). Scale bar = 100 µm. The data are represented as mean ± SEM.
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    ( A ) Schematic of AK7 knockdown in ACC glutamatergic neurons using shRNA. ( B ) Western blot confirming effective AK7 knockdown in the ACC (* P = 0.0407, two-sample Student’s t test, n = 3 mice per group). ( C ) Knockdown of AK7 significantly increased PWT and PWL in CFA mice (* P = 0.0154, ** P = 0.0028, two-sample Student’s t test, n = 6 mice per group). ( D ) Representative action potential firing traces elicited by 150 pA depolarizing current injection. Scale bars: 50 mV, 100 ms. ( E ) Statistical comparison of AP peak amplitude between the two groups ( P = 0.2986, no significant difference, two-sample Student’s t test, n = 7 neurons per group). ( F ) Input–output curve showing the number of evoked APs under gradient injected currents (0–300 pA) (*** P < 0.0001, two-way repeated-measures ANOVA). ( G ) Quantitative statistics of resting membrane potential (** P = 0.0087, two-sample Student’s t test, n = 7 neurons per group) and rheobase current (** P = 0.0067, two-sample Student’s t test, n = 7 neurons per group) of ACC neurons. ( H ) Representative raw traces of sEPSCs recorded from ACC neurons in Scramble (blue) and AK7-KD (red) groups. Scale bars: 20 pA, 1 s. ( I ) Statistical analysis of sEPSCs amplitude (left, * P = 0.0153, two-sample Student’s t test, n = 7 neurons per group) and frequency (right, ** P = 0.0011, two-sample Student’s t test, n = 7 neurons per group) between two groups. ( J ) Cumulative probability distribution curves of sEPSCs amplitude (left) and inter-event interval (right) in Scramble and AK7-KD groups. ( K ) Immunofluorescence showing reduced c-Fos expression in the ACC after AK7 knockdown (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( L – N ) Fiber photometry showing decreased Ca²⁺ activity in ACC neurons following AK7 knockdown (* P = 0.0481, ** P = 0.0027, two-sample Student’s t test, n = 6 per group). ( O ) The Western blot experiment confirmed the expression of p-AMPK protein in the ACC brain region of mice (* P = 0.0299; 0.0469, one-way ANOVA with Tukey’s multiple-comparisons test, n = 5 mice per group). ( P ) Biochemical quantitative detection results of adenosine concentration in the ACC brain region of mice (** P = 0.0042; 0.0027, one-way ANOVA with Tukey’s multiple-comparisons test, n = 6 mice per group). ( Q ) Biochemical quantitative detection results of <t>ATP/ADP</t> ratio in the ACC area of mice brain (* P = 0.0215; 0.0413, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 mice per group). ( R ) Schematic of AK7 overexpression in ACC glutamatergic neurons. ( S ) Western blot confirming AK7 overexpression (* P = 0.0364, two-sample Student’s t test, n = 4 mice per group). ( T ) Overexpression of AK7 decreased PWT and PWL in CON mice (** P = 0.0046; 0.0011, two-sample Student’s t test, n = 6 mice per group). ( U ) Increased ACC c-Fos expression following AK7 overexpression (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( V – X ) Fiber photometry showing enhanced Ca²⁺ activity in the ACC after AK7 overexpression (** P = 0.0052, *** P = 0.005, two-sample Student’s t test, n = 6 per group). Scale bar = 100 µm. The data are represented as mean ± SEM.
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    ( A ) Schematic of AK7 knockdown in ACC glutamatergic neurons using shRNA. ( B ) Western blot confirming effective AK7 knockdown in the ACC (* P = 0.0407, two-sample Student’s t test, n = 3 mice per group). ( C ) Knockdown of AK7 significantly increased PWT and PWL in CFA mice (* P = 0.0154, ** P = 0.0028, two-sample Student’s t test, n = 6 mice per group). ( D ) Representative action potential firing traces elicited by 150 pA depolarizing current injection. Scale bars: 50 mV, 100 ms. ( E ) Statistical comparison of AP peak amplitude between the two groups ( P = 0.2986, no significant difference, two-sample Student’s t test, n = 7 neurons per group). ( F ) Input–output curve showing the number of evoked APs under gradient injected currents (0–300 pA) (*** P < 0.0001, two-way repeated-measures ANOVA). ( G ) Quantitative statistics of resting membrane potential (** P = 0.0087, two-sample Student’s t test, n = 7 neurons per group) and rheobase current (** P = 0.0067, two-sample Student’s t test, n = 7 neurons per group) of ACC neurons. ( H ) Representative raw traces of sEPSCs recorded from ACC neurons in Scramble (blue) and AK7-KD (red) groups. Scale bars: 20 pA, 1 s. ( I ) Statistical analysis of sEPSCs amplitude (left, * P = 0.0153, two-sample Student’s t test, n = 7 neurons per group) and frequency (right, ** P = 0.0011, two-sample Student’s t test, n = 7 neurons per group) between two groups. ( J ) Cumulative probability distribution curves of sEPSCs amplitude (left) and inter-event interval (right) in Scramble and AK7-KD groups. ( K ) Immunofluorescence showing reduced c-Fos expression in the ACC after AK7 knockdown (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( L – N ) Fiber photometry showing decreased Ca²⁺ activity in ACC neurons following AK7 knockdown (* P = 0.0481, ** P = 0.0027, two-sample Student’s t test, n = 6 per group). ( O ) The Western blot experiment confirmed the expression of p-AMPK protein in the ACC brain region of mice (* P = 0.0299; 0.0469, one-way ANOVA with Tukey’s multiple-comparisons test, n = 5 mice per group). ( P ) Biochemical quantitative detection results of adenosine concentration in the ACC brain region of mice (** P = 0.0042; 0.0027, one-way ANOVA with Tukey’s multiple-comparisons test, n = 6 mice per group). ( Q ) Biochemical quantitative detection results of <t>ATP/ADP</t> ratio in the ACC area of mice brain (* P = 0.0215; 0.0413, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 mice per group). ( R ) Schematic of AK7 overexpression in ACC glutamatergic neurons. ( S ) Western blot confirming AK7 overexpression (* P = 0.0364, two-sample Student’s t test, n = 4 mice per group). ( T ) Overexpression of AK7 decreased PWT and PWL in CON mice (** P = 0.0046; 0.0011, two-sample Student’s t test, n = 6 mice per group). ( U ) Increased ACC c-Fos expression following AK7 overexpression (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( V – X ) Fiber photometry showing enhanced Ca²⁺ activity in the ACC after AK7 overexpression (** P = 0.0052, *** P = 0.005, two-sample Student’s t test, n = 6 per group). Scale bar = 100 µm. The data are represented as mean ± SEM.
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    Image Search Results


    ( A ) Schematic of AK7 knockdown in ACC glutamatergic neurons using shRNA. ( B ) Western blot confirming effective AK7 knockdown in the ACC (* P = 0.0407, two-sample Student’s t test, n = 3 mice per group). ( C ) Knockdown of AK7 significantly increased PWT and PWL in CFA mice (* P = 0.0154, ** P = 0.0028, two-sample Student’s t test, n = 6 mice per group). ( D ) Representative action potential firing traces elicited by 150 pA depolarizing current injection. Scale bars: 50 mV, 100 ms. ( E ) Statistical comparison of AP peak amplitude between the two groups ( P = 0.2986, no significant difference, two-sample Student’s t test, n = 7 neurons per group). ( F ) Input–output curve showing the number of evoked APs under gradient injected currents (0–300 pA) (*** P < 0.0001, two-way repeated-measures ANOVA). ( G ) Quantitative statistics of resting membrane potential (** P = 0.0087, two-sample Student’s t test, n = 7 neurons per group) and rheobase current (** P = 0.0067, two-sample Student’s t test, n = 7 neurons per group) of ACC neurons. ( H ) Representative raw traces of sEPSCs recorded from ACC neurons in Scramble (blue) and AK7-KD (red) groups. Scale bars: 20 pA, 1 s. ( I ) Statistical analysis of sEPSCs amplitude (left, * P = 0.0153, two-sample Student’s t test, n = 7 neurons per group) and frequency (right, ** P = 0.0011, two-sample Student’s t test, n = 7 neurons per group) between two groups. ( J ) Cumulative probability distribution curves of sEPSCs amplitude (left) and inter-event interval (right) in Scramble and AK7-KD groups. ( K ) Immunofluorescence showing reduced c-Fos expression in the ACC after AK7 knockdown (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( L – N ) Fiber photometry showing decreased Ca²⁺ activity in ACC neurons following AK7 knockdown (* P = 0.0481, ** P = 0.0027, two-sample Student’s t test, n = 6 per group). ( O ) The Western blot experiment confirmed the expression of p-AMPK protein in the ACC brain region of mice (* P = 0.0299; 0.0469, one-way ANOVA with Tukey’s multiple-comparisons test, n = 5 mice per group). ( P ) Biochemical quantitative detection results of adenosine concentration in the ACC brain region of mice (** P = 0.0042; 0.0027, one-way ANOVA with Tukey’s multiple-comparisons test, n = 6 mice per group). ( Q ) Biochemical quantitative detection results of ATP/ADP ratio in the ACC area of mice brain (* P = 0.0215; 0.0413, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 mice per group). ( R ) Schematic of AK7 overexpression in ACC glutamatergic neurons. ( S ) Western blot confirming AK7 overexpression (* P = 0.0364, two-sample Student’s t test, n = 4 mice per group). ( T ) Overexpression of AK7 decreased PWT and PWL in CON mice (** P = 0.0046; 0.0011, two-sample Student’s t test, n = 6 mice per group). ( U ) Increased ACC c-Fos expression following AK7 overexpression (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( V – X ) Fiber photometry showing enhanced Ca²⁺ activity in the ACC after AK7 overexpression (** P = 0.0052, *** P = 0.005, two-sample Student’s t test, n = 6 per group). Scale bar = 100 µm. The data are represented as mean ± SEM.

    Journal: EMBO Reports

    Article Title: Upregulation of AK7 in ACC-VTA glutamatergic projections contributes to chronic inflammation pain in male mice

    doi: 10.1038/s44319-026-00940-z

    Figure Lengend Snippet: ( A ) Schematic of AK7 knockdown in ACC glutamatergic neurons using shRNA. ( B ) Western blot confirming effective AK7 knockdown in the ACC (* P = 0.0407, two-sample Student’s t test, n = 3 mice per group). ( C ) Knockdown of AK7 significantly increased PWT and PWL in CFA mice (* P = 0.0154, ** P = 0.0028, two-sample Student’s t test, n = 6 mice per group). ( D ) Representative action potential firing traces elicited by 150 pA depolarizing current injection. Scale bars: 50 mV, 100 ms. ( E ) Statistical comparison of AP peak amplitude between the two groups ( P = 0.2986, no significant difference, two-sample Student’s t test, n = 7 neurons per group). ( F ) Input–output curve showing the number of evoked APs under gradient injected currents (0–300 pA) (*** P < 0.0001, two-way repeated-measures ANOVA). ( G ) Quantitative statistics of resting membrane potential (** P = 0.0087, two-sample Student’s t test, n = 7 neurons per group) and rheobase current (** P = 0.0067, two-sample Student’s t test, n = 7 neurons per group) of ACC neurons. ( H ) Representative raw traces of sEPSCs recorded from ACC neurons in Scramble (blue) and AK7-KD (red) groups. Scale bars: 20 pA, 1 s. ( I ) Statistical analysis of sEPSCs amplitude (left, * P = 0.0153, two-sample Student’s t test, n = 7 neurons per group) and frequency (right, ** P = 0.0011, two-sample Student’s t test, n = 7 neurons per group) between two groups. ( J ) Cumulative probability distribution curves of sEPSCs amplitude (left) and inter-event interval (right) in Scramble and AK7-KD groups. ( K ) Immunofluorescence showing reduced c-Fos expression in the ACC after AK7 knockdown (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( L – N ) Fiber photometry showing decreased Ca²⁺ activity in ACC neurons following AK7 knockdown (* P = 0.0481, ** P = 0.0027, two-sample Student’s t test, n = 6 per group). ( O ) The Western blot experiment confirmed the expression of p-AMPK protein in the ACC brain region of mice (* P = 0.0299; 0.0469, one-way ANOVA with Tukey’s multiple-comparisons test, n = 5 mice per group). ( P ) Biochemical quantitative detection results of adenosine concentration in the ACC brain region of mice (** P = 0.0042; 0.0027, one-way ANOVA with Tukey’s multiple-comparisons test, n = 6 mice per group). ( Q ) Biochemical quantitative detection results of ATP/ADP ratio in the ACC area of mice brain (* P = 0.0215; 0.0413, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 mice per group). ( R ) Schematic of AK7 overexpression in ACC glutamatergic neurons. ( S ) Western blot confirming AK7 overexpression (* P = 0.0364, two-sample Student’s t test, n = 4 mice per group). ( T ) Overexpression of AK7 decreased PWT and PWL in CON mice (** P = 0.0046; 0.0011, two-sample Student’s t test, n = 6 mice per group). ( U ) Increased ACC c-Fos expression following AK7 overexpression (*** P < 0.0001, one-way ANOVA with Tukey’s multiple-comparisons test, n = 4 brain sections from 4 mice per group). ( V – X ) Fiber photometry showing enhanced Ca²⁺ activity in the ACC after AK7 overexpression (** P = 0.0052, *** P = 0.005, two-sample Student’s t test, n = 6 per group). Scale bar = 100 µm. The data are represented as mean ± SEM.

    Article Snippet: ATP/ADP Ratio Chemiluminescence Assay Kit , Elabscience , E-BC-F004.

    Techniques: Knockdown, shRNA, Western Blot, Injection, Comparison, Membrane, Analysis, Immunofluorescence, Expressing, Activity Assay, Concentration Assay, Over Expression

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Upregulation of AK7 in ACC-VTA glutamatergic projections contributes to chronic inflammation pain in male mice

    doi: 10.1038/s44319-026-00940-z

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: ATP/ADP Ratio Chemiluminescence Assay Kit , Elabscience , E-BC-F004.

    Techniques: Chemiluminescence Immunoassay